whole cell protein extraction kit  (Sangon Biotech)

Journal: Experimental and Therapeutic Medicine

Article Title: Ginsenoside Rg1 promotes β‑amyloid peptide degradation through inhibition of the ERK/PPARγ phosphorylation pathway in an Alzheimer's disease neuronal model

doi: 10.3892/etm.2023.12319

Figure Lengend Snippet: Effect of ginsenoside Rg1 on ERK translocation in the neurons of an Alzheimer's disease model. (A) ERK immunofluorescence staining. Red represents ERK staining, whereas blue represents DAPI-stained nuclei (scale bar, 50 µm). (B) Western blotting was used to detect the nuclear and cytoplasmic protein expression levels of ERK. Comparisons of the nuclear and cytoplasmic protein expression levels of ERK, and the ratio of ERK protein expression in the nucleus to that in the cytoplasm (n=6). * P<0.05 vs. control group and # P<0.05 vs. model group.

Article Snippet: The neuronal nuclear protein, cytoplasmic protein and total protein fractions were extracted respectively using nuclear and cytoplasmic protein extraction and whole cell lysis assay kits (Wanleibio Co., Ltd.) according to the instructions of these kits.

Techniques: Translocation Assay, Immunofluorescence, Staining, Western Blot, Expressing, Control